Research and Development

       AIDS(Acquired Immunodeficiency Dyndrome) is an infectious disease caused by human immunodeficiency virus (HIV). T cells is the main type of cells which attacked by HIV virus. The typical Symptoms caused by HIV infection are dysfunction of patients’immune system, and eventually died of opportunistic infections or cancer. According to recent national reports, registered AIDS patients have already exceeded one million in China. 
       Due to the integration of virus genome and rapid variation of the viral protein, no specific medicine and vaccines have been developed So far. The most common treatment strategies to prolong the life of patients, such as HAART(highly active antiretroviral therapy), is to reduce viral load, maintain the number of T cells. However the treatment needs lifelong medication and is unable to get rid of latent virus. The gene therapeutic strategy, which can inhibit virus replication and eliminate the integrated virus genome from human genome, is a very promising way for AIDS treatment.
       In our HIV program, gene editing was employed as core technology for AIDS therapy. The key gene related to virus cycle was targeted to edite by CRISPR, so as to inhibit the virus replication.

名称

组织来源

种属

培养条件

生长特性

描述

293T

人肾上皮细胞系

Human

MEM/NEAA, 10%FBS

贴壁

经过腺病毒E1A基因的转染,能表达SV40大T抗原,含有SV40复制起始点与启动子区。

AAV-293

人肾上皮细胞系

Human

DMEM, 10% FBS

贴壁

293A来源于人胚肾,为293细胞的亚株,A为adhere意思,即293倾向形成单层细胞,其含有SV40启动子,适合于腺病毒的包装。

Hela

宫颈癌细胞

Human

MEM/NEAA, 10%FBS

贴壁

来自人体的组织培养中最早的分离细胞,经过人工培养、具有无限增殖能力,广泛应用于肿瘤研究、生物实验或者细胞培养。

HepG-2

人肝癌

Human

DMEM, 10% FBS

贴壁

来源于15岁白人的肝癌组织。

MCF-7

乳腺癌

Human

MEM/NEAA, 10% FBS10ug/ml 胰岛素

贴壁

保留乳腺上皮的特性。

MDA-MB-231

人乳腺癌细胞

Human

L15: Leibovitz Medium10%FBS

贴壁

MDA-MB-231是从一名51岁的白人女性乳腺癌患者的胸水中分离建立的。该细胞表达表皮生长因子EGF受体、TGF-α受体和WNT7B癌基因。

Hskmc

(BN)

人骨骼肌细胞株

Human

DMEM, 10% FBS

贴壁

国内细胞库,从流产的胎儿组织中提取后经培养训化而来,6-8代为最佳生长期。

Jurkat 

急性T淋巴细胞白血病

Human

RPMI-1640, 10%FBS

悬浮

用于研究急性T细胞白血病,T细胞信号传导以及易感病毒进入的各种趋化因子受体,特别是HIV的表达的永生化人T淋巴细胞系。

K562

骨髓性的白血病

Human

RPMI-1640, 10%FBS

悬浮

第一个人类髓性白血病人工培养的细胞,用于研究肿瘤和白血病治疗、药物靶标等领域。

MG-63

人骨肉瘤细胞

Human

MclCoy's SA(Modified)Medium

贴壁

来源于男性的骨肉瘤。

RD

人恶性横纹肌瘤细胞

Human

DMEM, 10% FBS

贴壁

来源于7岁女性盆腔横纹肌肉瘤,形态类似于原始的肿瘤纺锤体细胞和大的多核细胞。RD(ATCC®CCL-136)

RD139

人恶性横纹肌瘤细胞

Human

DMEM, 10% FBS

贴壁

来源于7岁女性盆腔横纹肌肉瘤,形态类似于原始的肿瘤纺锤体细胞和大的多核细胞。TE671(ATCC®HTB-139)

RD136

人恶性横纹肌瘤细胞

Human

DMEM(ATCC 30-2002), 10% FBS

贴壁

来源于7岁女性盆腔横纹肌肉瘤,形态类似于原始的肿瘤纺锤体细胞和大的多核细胞。RD(ATCC®CCL-136)

HaCat (Human Keratinocytes Cells)

人角质细胞

Human

DMEM(含2mM L-glutamine)+10%胎牛血清。冻存液:90%完全培养液,10%DMSO。现用现配。

贴壁

该细胞是来自于昆明细胞库的人皮肤角质细胞,细胞为贴壁生长,呈上皮细胞样。

KG-1

急性髓系细胞白血病细胞

Human

IMDM,10%FBS

悬浮

KG-1细胞系由H.P.Koeffler和D.W.Golde建立,骨髓抽自一个59岁的患有红白血病而后又发展成急性骨髓原性白血病的男性病人。

K-562

(CN)

人慢性髓系白血病细胞

Human

Human

悬浮

来自中国科学院细胞库,2019年新引进,细胞编号:SCSP-5054;连续细胞株 K-562 来源于一位临终的 53 岁女性慢性髓性白血病患者的胸水。 细胞被归入高度未分化的粒性白细胞类。

@ 2017 Guangdong Trement Medical Science and Technology Co., Ltd

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